When developing vaccines and cell and gene therapies, most people still rely on PCR and cell-based cloning for early development and later processing of genetically relevant information. However, these two are prone to limitations when cloning, including errors in the amplification cycle, time-consuming, unpredictable cytotoxicity, and the need to isolate the target genetic material, which is quite inconvenient. In contrast, the Cell-Free Cloning System developed by OriCiro is more efficient and comprehensive, which can replace traditional methods and speed up the drug development process. OriCiro can clone any sequence, including GC-rich, repetitive or cytotoxic sequences. Whether designing and constructing complex DNA templates in the early stage or manufacturing nucleic acid-based therapeutic reagents, this system can be used flexibly, which can not only reduce the cost and equipment required for experiments, but also do not need to worry about the safety of cell and gene therapy, application Wide range and great potential for development!