AccuGenomics’ patented and propriety technology, Standardized Nucleic Acid Quantification (SNAQ™) for Sequencing (SNAQ-SEQ) is used to manufacture Accukits™ that contain internal standard mixtures (ISM™) designed for a given set of genetic targets. These SNAQ-SEQ Spike-in standards are added to each specimen at the library prep stage of the workflow. Biochemically identical to the target regions except for the presence of a barcode (single base mutation) every 50-80 bp, each Internal Standard in the ISM will co-vary with their targets and allow measurement of background error rates due to technical or systematic errors known to occur throughout the sequencing process. The barcode signature contained within the sequence allows separate pipeline analysis of the target and ISM sequences.