- Gentest® Supersomes®
- 產品細節 Ⅰ
- 產品細節 ⅠⅠ
★ 高催化活性Supersomes® 的酵素活性通常比人類肝微粒體高出約 6 倍，有助於提高反應線性階段時間與產物生成效率，適合慢代謝化合物的深入研究。
★ 全面酵素組合提供最完整的一組 CYP 酵素，可覆蓋主要 Phase I 代謝途徑，並可延伸至 Phase II 路徑。
★ 模擬天然酵素動力學與人肝與其他器官天然酵素相比，Supersomes® 的酵素動力學表現一致，因此更接近 in vivo 代謝情況。
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優質重組 CYP 酵素 |
非 CYP 代謝酵素 |
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Supersomes® 含最完整的 CYP450 系列，可搭配 NADPH 還原酶，提供強大催化能力。
請詳見《產品細節》 |
除了 CYP，也提供以下重組酵素：
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常見用途（Applications）
✔ 高通量藥物交互作用篩選（High-throughput DDI screening） ✔ 慢代謝化合物代謝研究（Slow metabolizing compounds） ✔ 代謝物生成與結構鑑定（Metabolite identification） ✔ 反應表型分析（Reaction phenotyping） ✔ 酵素抑制與動力學分析（Enzyme inhibition & kinetics） ✔ 評估前體藥物（Pro-drug）與 CYP 代謝清除通路（CYP pathways）
All suitable for regulatory ADME studies and reaction phenotyping. |
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Supersomes® enable efficient metabolite formation with a long linear time course and high catalytic activity, making them ideal for screening slow-metabolizing drug compounds.
Human P450 Enzymes
|
Catalog Number |
Description |
P450 Content (nmol) |
Qty (mL) |
|
456211 |
Human CYP1A1 + reductase |
0.5 |
0.5 |
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456203 |
Human CYP1A2 + reductase |
0.5 |
0.5 |
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456220 |
Human CYP1B1 + reductase |
0.5 |
0.5 |
|
456204 |
Human CYP2A6 + reductase |
1 |
0.5 |
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456254 |
Human CYP2A6 + reductase + b5 |
0.5 |
0.5 |
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456210 |
Human CYP2B6 + reductase |
0.5 |
0.5 |
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456255 |
Human CYP2B6 + reductase + b5 |
0.5 |
0.5 |
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456222 |
Human CYP2C18 + reductase |
0.5 |
0.5 |
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456219 |
Human CYP2C19 + reductase |
0.5 |
0.5 |
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456259 |
Human CYP2C19 + reductase + b5 |
0.5 |
0.5 |
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456212 |
Human CYP2C8 + reductase |
1 |
0.5 |
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456252 |
Human CYP2C8 + reductase + b5 |
0.5 |
0.5 |
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456218 |
Human CYP2C9*1 (Arg144) + reductase |
1 |
0.5 |
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456258 |
Human CYP2C9*1 (Arg144) + reductase + b5 |
0.5 |
0.5 |
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456217 |
Human CYP2D6*1 (Val374) + reductase |
0.5 |
0.5 |
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456206 |
Human CYP2E1 + reductase + b5 |
1 |
0.5 |
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456264 |
Human CYP2J2 + reductase + b5 |
0.5 |
0.5 |
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456207 |
Human CYP3A4 + reductase |
1 |
0.5 |
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456202 |
Human CYP3A4 + reductase + b5 |
0.5 |
0.5 |
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456235 |
Human CYP3A5 + reductase |
1 |
0.5 |
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456256 |
Human CYP3A5 + reductase + b5 |
0.5 |
0.5 |
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456237 |
Human CYP3A7 + reductase + b5 |
0.5 |
0.5 |
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456221 |
Human CYP4A11 + reductase |
0.5 |
0.5 |
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456272 |
Human CYP4F2 + reductase + b5 |
0.5 |
0.5 |
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456273 |
Human CYP4F3A + reductase + b5 |
0.25 |
0.5 |
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456274 |
Human CYP4F3B + reductase + b5 |
0.5 |
0.5 |
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456275 |
Human CYP4F12 + reductase + b5 |
0.5 |
0.5 |
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456260 |
Human CYP19 (aromatase) + reductase |
0.5 |
0.5 |
Discovery also offers a range of non-CYP Supersomes® enzymes.
Human UGT Enzymes
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Catalog Number |
Description |
Protein Content (mg/mL) |
Qty (mL) |
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456400 |
UGT control microsomes |
5 |
0.5 |
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456411 |
Human UGT1A1 |
5 |
0.5 |
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456413 |
Human UGT1A3 |
5 |
0.5 |
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456414 |
Human UGT1A4 |
5 |
0.5 |
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456416 |
Human UGT1A6 |
5 |
0.5 |
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456407 |
Human UGT1A7 |
5 |
0.5 |
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456418 |
Human UGT1A8 |
5 |
0.5 |
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456419 |
Human UGT1A9 |
5 |
0.5 |
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456410 |
Human UGT1A10 |
5 |
0.5 |
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456424 |
Human UGT2B4 |
5 |
0.5 |
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456427 |
Human UGT2B7 |
5 |
0.5 |
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453323 |
Human UGT2B10 |
5 |
0.5 |
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456435 |
Human UGT2B15 |
5 |
0.5 |
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456437 |
Human UGT2B17 |
5 |
0.5 |
UGT = UDP-glucuronosyltransferase
Other Human Metabolic Enzymes
|
Catalog Number |
Description |
Protein Content (mg/mL) |
Qty (mL) |
| 453320 | Human CES1b |
5 |
0.5 |
| 453321 | Human CES1c |
5 |
0.5 |
| 453322 | Human CES2 |
5 |
0.5 |
| 453322 | Human FMO1 |
5 |
0.5 |
| 456233 | Human FM03 |
5 |
0.5 |
| 456245 | Human FM05 |
5 |
0.5 |
| 456280 | Monoamine Oxidase (MAO) Control |
5 |
0.5 |
| 456283 | Human Monoamine Oxidase A (MAO-A) |
5 |
0.5 |
| 456284 | Human Monoamine Oxidase B (MAO-B) |
5 |
0.5 |
| 456800 | Human Control Cystosol |
5 |
0.5 |
| 456801 | Human Aldehyde Oxidase (AOX1) |
5 |
0.5 |
FMO = Flavin-containing monooxygenase
Gentest® Supersomes® Controls, Cofactors, and Buffers
Analysis of Cytochrome P450, UGT, and MAO isoform-specific metabolism requires use of the appropriate control preparation in order to eliminate the possibility of metabolism by enzymes native to the cell lines.
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Catalog Number |
Supersomes®Descriptions |
Protein Concentration (mg/mL) |
Qty(mL) |
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456200 |
Insect cell control for Gentest® Supersomes® enzymes baculovirus-insect cell-expressed (BTl-TN-5B1-4 cells) |
5 |
1.5 |
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456201 |
Insect cell control for Gentest® Supersomes® enzymes baculovirus-insect cell-expressed (BTl-TN-5B1-4 cells) |
5 |
0.5 |
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456400 |
Human UGT Insect Control for Gentest® UGT-expressing Supersomes® |
5 |
0.5 |
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456280 |
MAO Insect Control for Gentest® MAO-expressing Supersomes® |
5 |
0.5 |
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456244 |
Human P450 reductase and b5 control for baculovirus expressed (BTl-TN-5B1-4 cells) |
5 |
0.5 |
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Catalog Number |
Cofactor Description |
Qty(mL) |
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451220 |
NADPH Regenerating system — Solution A (NADP+ and GIc-6-PO4) |
5 |
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451200 |
NADPH Regenerating system — Solution B (G6PDH) |
1 |
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451300 |
UGT Reaction mix — Solution A (UDPGA) |
2 |
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451320 |
UGT Reaction mix — Solution B (Alamethicin) |
5 |
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Catalog Number |
Buffer Description |
Qty(mL) |
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451201 |
Phosphate buffer (pH 7.4) |
500 |
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